Effect of Cellulose Powder in a Mouse Model of Dextran Sodium Sulfate- Induced Colitis Jun SHIMIZU*, Hitomi KAMIYA, Kazuo OHTAKE, Hiroyuki UCHIDA, Jun KOBAYASHI, Hiroshi MANO Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University | Dietary fiber influences the course of ulcerative colitis, however, the effects of fiber quantity and water solubility are not fully established. We investigated the effects of insoluble and soluble dietary fiber in mice with dextran sodium sulfate (DSS)-induced colitis. Mice with DSS-induced colitis were fed a fiber-free diet (control), and diets containing either 5% cellulose powder (CP), 2.5% CP + 2.5% pectin (PE), or 5% PE. The disease activity index (DAI) in the 5% CP group was significantly lower compared to values in the other three groups. In mice fed the 5% CP diet, goblet cells disappeared from colon tissue, and neutrophils infiltrated this area. Myeloperoxidase activity and expression of inflammation-related genes in colon tissue were decreased in mice fed the 5% CP diet. Further studies were performed in which mice were fed a diet containing 5% or 10% CP during DSS administration. DAI, myeloperoxidase activity, and inflammatory gene expression levels in colon tissue were suppressed in mice fed the 10% CP diet. These results suggest that PE was ineffective in a DSS-induced colitis mouse model. However, these findings are not applicable to all water-soluble dietary fibers. Furthermore, the ingestion of CP exerted an anti-inflammatory effect via the fecal bulking effect within the colon. |
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