ラットを1,5- アンヒドログルシトール飼料で飼育したときの成長，臓器重量，血液性状ならびに酸化ストレスなどに及ぼす影響 中村禎子1，田辺賢一2，宮原明美3，泉千代子4，
吉永一浩4，山崎優子1，佐々木菜穂1，奥 恒行1, 3, 5＊ 1十文字学園女子大学食・栄養・健康研究所
2名古屋女子大学家政学部
3長崎県立大学大学院シーボルト校人間健康科学研究科
4日本澱粉工業（株）新素材商品開発室
5保健栄養・糖質科学ラボ 1,5-anhydroglucitol (1,5-AG) has 60% of the sweetness of sucrose, and is found in low levels in natural plantfoods, with small amounts being synthesized in the body. 1,5-AG is used as a marker of blood glucose control in patients with diabetes mellitus. Male Wistar rats aged 4-wk were raised using consecutive feeding of diets containing 5% and 10% of 1,5-AG for 31 days. Erythritol, which is easily absorbed from the small intestine and excreted in the urine without degradation, and fructooligosaccharide, which is not digested by small intestinal enzymes and is fermented completely by intestinal microbes were used as controls in the study. The growth and organ weights of the rats were not significantly different between the control group and the other 4 groups (n=5 in each group), because the amount of dietary intake was adjusted to become identical by light restricted feeding. In the experiment of revenue and expenditure, almost 100% of the 1,5-AG ingested was recovered from urine collected for 24 h. These results demonstrate that almost all of I,5-AG ingested is absorbed from the small intestine and is excreted in the urine without degradation, with the available energy being 0 kcal/g. 1,5-AG did not affect the concentration of blood glucose or triacylglycerol following consecutive feeding for 31 days and also did not induce morphological abnormalities in organs and tissues with no cecal enlargement in the fructooligosaccharide (FOS) group. The anti-oxidative potential and oxidative index of plasma and the amount of 8-hydroxy-2'-deoxyguanosine (8OHdG) excreted in the urine were not significantly different between the five groups. These results suggest that ingestion of 1.6 g of 1,5-AG/kg body weight per day (average intake for 31 days) does not have harmful effects on the growth and physical condition of rats. Taken together these results demonstrate that 1,5-AG is a safe ingredient in food.
Key words: 1,5-anhydroglucitol, growth, organ weight, blood biochemical marker, oxidative stress |
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