大麦若葉末摂取による1, 2-dimethylhydrazine誘発マウス異型陰窩巣（ACF）の抑制効果 片山（須川）洋子1＊，奥 和之1, 5，片山眞之1，
島田良子1，山口容子2，村上 香3,
神谷智康4，池口主弥4 1大阪青山大学健康栄養学科
2福岡女子大学環境科学科
3広島工業大学食品生命科学科
4株式会社東洋新薬研究開発本部
5川崎医療福祉大学臨床栄養学科
The inhibitory effects of dietary supplementation with young barley leaf powder (YBLP) on 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) formation in the colon were investigated in mice. Male BALC/c mice were fed a semi-purified AIN-93G diet containing 3% cellulose (Control-diet) or 3% cellulose plus 3% YBLP (YBLP-diet) for 17 weeks. Mice were divided into 4 treatment groups: (i) DMH(−) - Control-diet, (ii) DMH(−) - YBLP-diet, (iii) DMH(+) - Control-diet, and (iv) DMH(+) - YBLP-diet. Mice in the DMH(+) groups were intraperitoneally injected with DMH at a dose of 20 mg/kg body weight every week for 10 weeks, while those in the DMH(−) groups were not injected with DMH. The gastrointestinal transit time was shortened in the mice fed the YBLP-diet. The number and frequency of ACF in the colon were lowered significantly in the DMH-treated mice fed the YBLP-diet in comparison with those fed the Control-diet. The serum 8-hydroxy-2′-deoxyguanosine concentration decreased significantly in the DMH-treated mice fed the YBLP-diet. As for the levels of organic acids in the cecal contents, the DMH-treated mice fed the YBLP-diet displayed significantly increased levels of lactic acid, acetic acid, propionic acid, and n-butyric acid, compared with the group fed the Control-diet. The above results indicate that the YBLP-diet may have a suppressive effect on the induction of colonic ACF by DMH injection, suggesting suppression of the induction of colonic cancer by DMH administration.
Key words : young barley leaf powder, 1,2-dimethylhydrazine, mice, aberrant crypt foci, 8-hydroxy-2’- deoxyguanosine, organic acids, transit time. |
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