乳酸菌Leuconostoc mesenteroides NTM048株が産生する菌体外多糖は乾癬モデルマウスの症状を軽減する 畑堀 翔 1，中野智裕 1，久 景子 2，米島靖記 2，鈴木卓弥 1, 3 * 1 広島大学大学院生物圏科学研究科
2 日東薬品工業株式会社
3 広島大学大学院統合生命科学研究科 Psoriasis is a chronic infiammatory skin disorder characterized by epidermal hyperproliferation and abnormal differentiation. Interleukin-17 (IL-17), which is produced both systemically and topically, plays a crucial role in the pathogenesis of psoriasis. Leucosaccharide (LS) is an exopolysaccharide produced by the Leuconostoc mesenteroides strain NTM048. This study examined the ameliorative effect of LS in a murine model of imiquimod-induced psoriasis. Mice were fed diets without or with 0.7%, 1.7%, and 3.3% LS for 27 days and topically treated with an imiquimod cream for 6 days from the 21st day after feeding initiation. This treatment induced erythema as well as thickening and scaling of the dorsal skin in these mice. Supplemental LS reduced the clinical score of psoriasis on the 27th day in a dose-dependent manner. Although IL-17 production increased in the mice splenocytes as a result of the treatment, it decreased in the mice that fed the 1.7% and 3.3% LS diets. The levels of acetic, propionic, and n-butyric acids were higher in the cecum of mice that fed the 3.3% LS diet than in those that were not fed with this diet. Real-time quantitative polymerase chain reaction revealed that the 3.3% LS diet mitigated imiquimod-induced effects, with an increase in the cecal levels of Akkermansia muciniphila. These findings suggested that IL-17 suppression in splenocytes leads to LS-mediated reduction of psoriasis. Although the role of short-chain fatty acids (SCFA) in the IL-17 suppression is unclear, the A. muciniphila abundance may partially contribute to SCFA production in LS-fed mice.
Key words: exopolysaccharide, inteleukin-17, Leuconostoc mesenteroides, microbiota, psoriasis |
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