Estimation of the Digestibility ofIsomaltooligosaccharide
and Its Hydrogenated Derivative Using the Modified
Prosky Method (Enzymatic-LC method) Jun TSUNEHIRO1, ShoujiAWAMOTO1, Toshiyuki KANEKO2, and TsuneyaYATAKE2 1Research and Development Center, Kashima Laboratory,Showa Sangyo Co. Ltd.
2Research and Development Center, Showa Sangyo Co.Ltd. The digestibility of oligosaccharides was studiedby the modified Prosky method (Enzymatic-LC method), which wasofficially adopted the determination of low-molecular solubledietary fiber. When the low-digestible saccharide,isomaltooligosaccharide (IMO) and its hydrogenated derivative(IMO-H) were applied to the methods, the hydrolysis ratios wererelatively lower than those of starch hydrolysate, although theratios depended on the concentrations of enzyme and substrate.Changes in saccharides composition of IMO or IMO-H were almost thesame as those which were observed in our previous studies usingintestinal mucosa enzyme. These results suggested that theEnzymatic-LC method could be useful for estimation of digestibilityfor some kinds of oligosaccharide, derived from starch. Furtherimprovements of the enzymatic digestion system, which couldsimulate a human gastric enzyme mixture, will be able to establishan available method for various types of oligosaccharides.
Key word: digestibility, enzymatic-LC method,isomaltooligosaccharide, hydrogenated isomaltooligosaccharide,gastric enzyme |
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