Review

Dietary Fiber: Determination Methods andDefinition

Bunpei MORI

Toita Women's College, Emeritus Professor.

Relationships between analytical methods and thedefinition of dietary fiber are reviewed with analytical history,and discussed on the basis of results of the authors and theircolleagues.
1) The review discussed on protein-nitrogen and noneprotein-nitrogen which reduced as an undigestible material throughProsky method. Therefore, a modified determination method includingan undigestible ma-terial after enzyme hydrolysis, was proposed inthis review.
2) The analytical process for Van Soest, Southgate, Asp, Prosky,modified Prosky Englyst and Upsala method respectively, discussedusing each sample or as a model.
3) Resistant starch (RS) was prepared with pancreatin amylasedigestion completely, but it dissolved about 50% by the digestionof Prosky method. Therefore, a new analytical method of RS may benecessary except Termamyl digestion.
4) Relationship between enzymatic method and HPLC procedure forEtOH soluble DF were discussed.

Key words: dietary fiber, determination method, resistantstarch

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