Study On the Preventive MechaniSnl ofOligosaccharide FragmentS from Corn Bran HemicelluIoSe againstD-galactosamine-induced Hepatitis in Rats Xin Geng, Xiaokun Wang, Yukari Egashira and HirooSanada
Laboratory of Food and Nutrition, Graduate School of Science andTechnology, Chiba University
| In the present study, the effect ofoligosaccharide fragments (MW 286-930) obtained enzymatically fromcorn bran lclnicellulose (CBH), a soluble dietary fiber, on thedevelopment of hepatitis induced by D-galactosamine wasinvestigated, Seventy-two male Wistar rats that were fed a dietsupplemented with the oligosaccharide fragments were administeredD-galactosamine intraperitoneally at a dose of 500 mg/kg andeuthanized just before injection (O h) and 1, 3, 9, 16 and 22 hafter injection. Plasma endotoxin concentrations, serum cytokinelevels, and serum transaminase activities were analyzed lr dditionto liver DNA fragmentation. The results showed that theoligosaccharide fragments significantly suppressed thr ncrease inplasma endotoxin concentrations and significantly depressed theenhancement of serum transaminase activities Ind tumor necrosisfactor-α (TNF-α) but showed no effect on seruminterferon-γ and liver DNA fragmentation. These resultssuggested that the oligosaccharide fragments obtained enzymaticallyfrom CBH might protect the liver against D-galalctosamine hepatitisthrough the suppression of both plasma endotoxin increment andserum TNF-α production. |
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