Originals

Chemical Characteristics and HistochemicalObservations On Dietary
Fiber of Persimmon (Diospyros Kaki Linn. f. ) Leaves

Kikue KUSUNOKI1, TakayukiHARA1, Mamoru FUJITA1, HiroshiISHIDA2,
Miki MATSUGUMA1, Keita KUDOH3, YoshimiMINARI1, Noritaka TOKUI4,
Tadahiro TADOKORO5, Akio MAEKAWA5, andSatoshi INNAMI5*

1Faculty of Nutritional Sciences, Nakamura GakuenUniversity
2Department of Nutrition, College of Tokyo University ofAgriculture
3Department of Nutrition, Faculty of Health and Welfare,Nayoro City University
4Institute of Industrial Ecological Science, Universityof Occupational and Environmental Health
5Facalty of Applied Biological Science, Tokyo Universityof Agriculture

 Characteristics of dietary fiber in persimmon leaves wereinvestigated by using analytochemical and histochemical techniques.The staining examination with the various labeled lectins for freshpersimmon leaves showed high affinity for the specific sugarresidues or sugar chains in hemicellulose, pectic substance andglycoprotein constituting the cell wall of leaves. The sugarcomposition analysis and histochemical observation showed that theinsoluble hemicellulose fraction may be hetero-polysaccharideconsisted of arabinose as main sugar chain, and xylose andgalactose as side chain. The results also revealed that the solublehemicellulose fraction may be categorized to acidichetro-polysaccharide composed of galacturonan as main sugar chainand mannose and arabinose as side chain. The two fractions ofsoluble acidicpolysaccharide with different uronic acid contentswere found to belong to pectic substances. The present resultsmay
indicate that some significant informations were presented on theproperties of hemicellulose and pectic substance referring tomatrix structure of cell wall in persimmon leaves.

Key words: persimmon leaves, dietary fiber, acidic polysaccharides,histochemical observation, lectin, pectic substance.


Return