性状の異なる難消化吸収性オリゴ糖および糖アルコールの腸内細菌を介した生体利用性―抗菌剤による除菌ラットを用いた検討 ― 中村禎子1, 2, 3，福島恭子3，田辺賢一2, 3, 4，志村二三夫1，奥 恒行1, 2, 3 1十文字学園女子大，食・栄養・健康研究所，人間生活学部食物栄養学科，
2長崎県立大・大学院・人間健康科学研究科，
3長崎県立大学シーボルト校 看護栄養学部栄養健康学科，
4名古屋女子大学 家政学部食物栄養学科 | Providing rats with drinking water that contained three kinds of antibiotics for fourteen days resulted in significant, though incomplete depletion of intestinal microbes. The weights of cecal tissue and content increased significantly in antibiotics-treated rats (anti-rats) as compared to conventional rats (conv-rats). To compare the fermentability of nondigestible and/or nonabsorbable saccharides, several saccharides with different properties were cultivated under anaerobic conditions with cecal contents of anti-rats (CA) and conv-rats (CC) at 23-25℃ for 6 h using glucose as a reference. When sucrose was cultivated with CC, pH declined from 7.0 to 6.0, production of short-chain fatty acids (SCFAs) increased, and sucrose content decreased. These changes were significantly less than those of glucose (p<0.05). When lactitol and mannitol were cultivated with CC for 6 h, pH decline, SCFAs production, and substrate decrease were scarcely detectable. For fructooligosaccharide (FOS), the phenomenon was moderate between those of glucose and lactitol. However, when CA was used in cultivation with these saccharides, pH decline and SCFAs production were not observed in any test substances, except sucrose. When FOS or lactitol (200 mg/2 mL) was orally administered to conv-rats and anti-rats, the amount of FOS and lactitol recovered from the cecum and colon of anti-rats was minimal (5-11% ), yet was still significantly greater than those in conv-rats. These results demonstrate that fermentability of nondigestible and/or nonabsorbable oligosaccharide and sugar alcohol vary from saccharide to saccharide, and are utilized via intestinal microbes in rats. Key words: anitibiotics-treated rat, conventional rat, intestinal microbes, fermentability, nondigestible and/or nonabsorbable saccharide. |
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